Current issues of ACP Journal Club are published in Annals of Internal Medicine


Intranasal, live, attenuated influenza virus vaccine reduced severe febrile illness in healthy adults


ACP J Club. 2000 Mar-Apr;132:49. doi:10.7326/ACPJC-2000-132-2-049

Source Citation

Nichol KL, Mendelman PM, Mallon KP, et al., for the Live Attenuated Influenza Virus Vaccine in Healthy Adults Trial Group. Effectiveness of live, attenuated intranasal influenza virus vaccine in healthy, working adults: a randomized controlled trial. JAMA. 1999 Jul 14;282:137-44. [PubMed ID: 10411194]



Is an intranasal, trivalent, live, attenuated influenza virus (LAIV) vaccine effective and safe for reducing febrile illness in healthy, working adults?


Randomized (allocation not concealed*), blinded (participants and study personnel),* placebo-controlled trial with follow-up during site-specific 7-week peak outbreak periods.


13 sites in the continental United States.


4561 persons (mean age 38 y, 55% women) who were 18 to 64 years of age, worked ≥ 30 h/wk outside of the home, and had health insurance. Exclusion criteria included hypersensitivity to eggs, indication for or previous receipt of the inactivated vaccine, and pregnancy or unprotected risk for pregnancy in the previous 3 months. Follow-up was 93%.


From September 18 to November 15, 1997, 3041 participants received the LAIV vaccine (A/Shenzhen/227/95 [HINI], A/Wuhan/359/95 [H3N2], and B/Harbin/7/94-like) administered in a single-dose nasal spray. 1520 participants received the placebo vaccine.

Main outcome measures

3 illness categories were defined: febrile illness (≥ 2 consecutive d of symptoms, with fever and ≥ 2 symptoms each for ≥ 1 d); severe febrile illness (≥ 3 consecutive d of symptoms, with ≥ 1 d of fever and ≥ 2 symptoms for ≥ 3 d); and febrile upper respiratory tract infection (URTI) (≥ 2 consecutive d of URTI symptoms, fever for ≥ 1 d, and 2 symptoms for ≥ 1 d). Outcomes for each illness category included number of illness episodes, days of illness, days of missed work, and days with ≥ 1 health care provider visit.

Main results

The groups did not differ for febrile illness episodes (P = 0.10), but the vaccine group had lower rates of severe febrile illness episodes and related outcomes (Table) and lower rates of febrile URTI episodes (23.6% reduction in rates/1000, 95% CI 12.7 to 33.2) and related outcomes. No serious adverse effects occurred in either group.


Intranasal, trivalent, live, attenuated influenza virus vaccine reduced severe febrile illness and outcomes related to febrile upper respiratory tract illness in healthy, working adults.

*See Glossary.

Source of funding: Aviron.

For correspondence: Dr K.L. Nichol, Medicine Service (111), Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA. FAX 612-727-5659.

Table. Severe febrile illness outcomes in healthy, working adults receiving influenza vaccine vs placebo

Outcomes during 7-wk peak outbreaks Rate/1000 persons Rate reduction (95% CI)
Vaccine Placebo
Number of illness episodes 111 137 18.8% (7.4 to 28.8)
Days of illness 1021 1404 27.3% (16.7 to 36.5)
Days of missed work 155 188 17.9% (4.3 to 29.5)
Days with ≥ 1 health care visit 37.6 50.1 24.8% (11.6 to 36.1)


The limitations of the current inactivated influenza vaccine include need for an annual injection, poor induction of mucosal and cellular immune responses, and variable effectiveness, especially in such high-risk groups as the elderly.

After intranasal administration, LAIV replicates, producing a mild sore throat or coryza and inducing serum IgG and mucosal IgA anti-influenza antibodies. Person-to-person spread of LAIV does not seem to occur, and there seems to be no risk for reversion to a wild-type phenotype (1).

Nichol and colleagues showed that intranasal, trivalent LAIV was safe and effective in healthy adults aged 18 to 64 years. The primary end point was febrile illnesses during influenza outbreak periods, which tends to underestimate the vaccine’s true efficacy. For example, in children, the LAIV vaccine was 93% effective against culture-confirmed influenza but only 25% to 30% effective in reducing febrile episodes (2).

Unlike inactivated vaccine, which induces type-specific IgG antibodies, the broader protection of the LAIV vaccine might result from induction of cross-reactive mucosal IgA antibodies (1). A study that directly compared a bivalent LAIV with inactivated vaccines, however, did not show any advantage for LAIV vaccine when the circulating influenza virus was a poor antigenic match (3). No data are published on its use in immunocompromised patients; in older persons, it will probably be used as a supplement to the inactivated vaccine (4).

The LAIV vaccine will probably be licensed within the next year. In addition to its potential immunologic advantage, it might also have higher patient acceptability: It does not require a needle, and about two thirds of the recipients readily self-administered LAIV vaccine. Consumers can expect to pay for this convenience.

Bradley S. Bender, MD
Veterans Affairs Medical Center
Gainesville, Florida, USA


1. Subbarao K. Influenza vaccines: present and future. Adv Virus Res. 1999;54:349-73.

2. Belshe RB, Mendelman PM, Treanor J, et al. The efficacy of live attenuated, cold-adapted, trivalent, intranasal influenza virus vaccine in children. N Engl J Med. 1998;338:1405-12.

3. Edwards KM, Dupont WD, Westrich MK, et al. A randomized controlled trial of cold-adapted and inactivated vaccines for the prevention of influenza A disease. J Infect Dis. 1994;169:625-33.

4. Treanor JJ, Mattison HR, Dumyati G, et al. Protective efficacy of combined live intranasal and inactivated influenza A virus vaccines in the elderly. Ann Intern Med. 1992;117:625-33.